Expression of Bax, Bad and Bcl-2 proteins under x-radiation effect towards human breast carcinoma MCF-7 cells and their doxorubicin-resistant derivatives
| Autor: | I V, Chorna, L O, Datsyuk, R S, Stoika |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Antibiotics
Antineoplastic DNA Repair Carcinoma Breast Neoplasms Gene Expression Regulation Neoplastic Proto-Oncogene Proteins c-bcl-2 Doxorubicin Drug Resistance Neoplasm Tumor Cells Cultured Humans Electrophoresis Polyacrylamide Gel bcl-Associated Death Protein Comet Assay DNA Damage bcl-2-Associated X Protein |
| Zdroj: | Experimental oncology. 27(3) |
| ISSN: | 1812-9269 |
| Popis: | 1) To investigate the effect of X-rays on expression of Bax, Bad, and Bcl-2 proteins in human breast carcinoma MCF-7 cells, sensitive (MCF-7(wt)) and resistant (MCF-7(DOX/R)) to doxorubicin; 2) to measure X-radiation-induced DNA damage in order to clarify if changes in the expression of those proteins and DNA repair capacity can be responsible for the development of drug-resistance and for altered radio-sensitivity of MCF-7(DOX/R) cells.Expression of Bax, Bad and Bcl-2 proteins was detected by Western-blot analysis. DNA damage induced by X-radiation and the kinetics of subsequent DNA repair were estimated by alkaline DNA comet assay.We found an increase in the expression of pro-apoptotic Bax and Bad proteins and a decrease in the expression of Bcl-2 protein in MCF-7(wt) cells under the effect of X-radiation, whereas the expression of those proteins was not changed significantly in the irradiated MCF-7(DOX/R) cells. Different pattern of Bcl-2 expression was shown in MCF-7(DOX/R) cells compared with MCF-7(wt) cells. A decreased level of X-radiation-induced DNA damage and an increased DNA repair capacity were revealed in MCF-7(DOX/R) cells compared with MCF-7(wt) cells.The results of our study suggest that a reduced apoptotic response and an increased DNA repair capacity in MCF-7(DOX/R) cells might contribute to the resistance of these cells to X-radiation treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|