| Autor: |
R, Moats, L Q, Ma, R, Wajed, Y, Sugiura, A, Lazaryev, M, Tyszka, R, Jacobs, S, Fraser, M D, Nelson, Y A, DeClerck |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Clinicalexperimental metastasis. 18(6) |
| ISSN: |
0262-0898 |
| Popis: |
Neuroblastoma is the second most common solid tumor in children. So far few tumor models for this cancer have been reported in mice. We have created a murine tumor model for studying human neuroblastoma based on surgical orthotopic implantation in scid mice. Small fragments of subcutaneous tumors of SK-N-BE(2) human neuroblastoma cells expressing enhanced green fluorescent protein were surgically implanted near the left adrenal gland of scid mice. One hundred percent of the animals (n = 21) successfully implanted developed a large retroperitoneal tumor and became moribund between 22 and 57 days after implantation (mean survival time = 41 days). At the time of sacrifice the presence of bone marrow metastasis was detected by RT-PCR for green fluorescent protein in 95% of the cases. The growth of small tumor implants could be easily visualized and quantified by surveillance MR imaging, with a resolution of 117 x 117 x 750 microm in two orthogonal planes allowing accurate volume measurements, as well as assessment of necrosis and tissue invasion. This novel model should be a valuable tool to study the biology and therapeutic approaches to neuroblastoma. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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