| Autor: |
Yan-Yuan, Qian, Li-Li, Liu, Xiao-Juan, Yu, Cong, Ding, Zhi-Ping, Wang, Yu-Heng, Shi, Chen-Jun, Li |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Huan jing ke xue= Huanjing kexue. 33(11) |
| ISSN: |
0250-3301 |
| Popis: |
In order to investigate the characteristics of the microbial degradation of tetrabromobisphenol A (TBBPA), four aerobic strains were isolated from the phenol granular sludge using selective medium with TBBPA as the sole carbon source. Among them, strain H could accommodate the TBBPA culture medium and degrade TBBPA with high efficiency, which was identified as Rhodococcus sp. via DNA sequencing test. The optimal conditions for TBBPA degradation by strain H was pH 6.5, 30 degrees C, 150 r x min(-1), the concentration of humic acid being 200 mg x L(-1) and the concentration of K+ being 1 000 mg x L(-1). Under the optimal conditions, the debromination rate of TBBPA reached 20.75% after 21 d. The results of LC-MS showed that the major degradation product was monobromophenol, which was formed by the removal of isopropyl from the benzene ring in TBBPA molecule. The results of protein gel electrophoresis (SDS-PAGE) showed that strain H had a protein band between (90-117) x 10(3) kDa, which might be the enzyme responsible for TBBPA degradation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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