| Autor: |
A J, Madonna, F, Basile, I, Ferrer, M A, Meetani, J C, Rees, K J, Voorhees |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Rapid communications in mass spectrometry : RCM. 14(23) |
| ISSN: |
0951-4198 |
| Popis: |
A rapid methodology is described for the enhancement of the signal-to-base-line (S/B) ratio of high molecular weight protein signals from whole cell bacteria analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The procedure involves depositing growing bacteria colonies from culture dishes directly onto the MALDI probe followed by treatment of the sample spot with a 2 microL aliquot of 40% ethanol prior to the addition of a ferulic acid matrix solution (12.5 mg dissolved in 17% formic acid/33% acetonitrile/50% H(2)O). Protein signals of more than 20 kDa were routinely produced from both Gram positive and Gram negative bacteria prepared in this manner. Moreover, a substantial number of intense protein signals were also produced in the more 'conventional' fingerprint region extending from 4 to 20 kDa. This approach is rapid, easy to implement into existing methodologies, and does not require any special hardware. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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