| Autor: |
Bárbara B, Sousa, Marcos F Q, Sousa, Marta C, Marques, João D, Seixas, José A, Brito, Pedro M, Matias, Gonçalo J L, Bernardes, António, Roldão |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Bioprocess and biosystems engineering. 44(1) |
| ISSN: |
1615-7605 |
| Popis: |
Bone Marrow Tyrosine kinase in the chromosome X (BMX) is a TEC family kinase associated with numerous pathological pathways in cancer cells. Covalent inhibition of BMX activity holds promise as a therapeutic approach against cancer. To screen for potent and selective covalent BMX inhibitors, large quantities of highly pure BMX are normally required which is challenging with the currently available production and purification processes. Here, we developed a scalable production process for the human recombinant BMX (hrBMX) using the insect cell-baculovirus expression vector system. Comparable expression levels were obtained in small-scale shake flasks (13 mL) and in stirred-tank bioreactors (STB, 5 L). A two-step chromatographic-based process was implemented, reducing purification times by 75% when compared to traditional processes, while maintaining hrBMX stability. The final production yield was 24 mg of purified hrBMX per litter of cell culture, with a purity of 99%. Product quality was assessed and confirmed through a series of biochemical and biophysical assays, including circular dichroism and dynamic light scattering. Overall, the platform herein developed was capable of generating 100 mg purified hrBMX from 5 L STB in just 34 days, thus having the potential to assist in-vitro covalent ligand high-throughput screening for BMX activity inhibition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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