Coupling of Rigor Mortis and Intestinal Necrosis during C. elegans Organismal Death
| Autor: | Evgeniy R, Galimov, Rosina E, Pryor, Sarah E, Poole, Alexandre, Benedetto, Zachary, Pincus, David, Gems |
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| Rok vydání: | 2017 |
| Předmět: |
Aging
calcium muscle Muscles Rigor Mortis C. elegans Models Biological Receptor Insulin Fluorescence Article necrosis Death Intestines ATP Adenosine Triphosphate organismal death Mutation Animals Insulin pathology Calcium Signaling Insulin-Like Growth Factor I Caenorhabditis elegans Caenorhabditis elegans Proteins Muscle Contraction |
| Zdroj: | Cell Reports |
| ISSN: | 2211-1247 |
| Popis: | Summary Organismal death is a process of systemic collapse whose mechanisms are less well understood than those of cell death. We previously reported that death in C. elegans is accompanied by a calcium-propagated wave of intestinal necrosis, marked by a wave of blue autofluorescence (death fluorescence). Here, we describe another feature of organismal death, a wave of body wall muscle contraction, or death contraction (DC). This phenomenon is accompanied by a wave of intramuscular Ca2+ release and, subsequently, of intestinal necrosis. Correlation of directions of the DC and intestinal necrosis waves implies coupling of these death processes. Long-lived insulin/IGF-1-signaling mutants show reduced DC and delayed intestinal necrosis, suggesting possible resistance to organismal death. DC resembles mammalian rigor mortis, a postmortem necrosis-related process in which Ca2+ influx promotes muscle hyper-contraction. In contrast to mammals, DC is an early rather than a late event in C. elegans organismal death. Video Abstract Graphical Abstract Highlights • A wave of body wall muscle contraction occurs during C. elegans organismal death • This rigor mortis-like phenomenon is coupled to a wave of intestinal necrosis • Both waves are accompanied by Ca2+ release and a drop in ATP levels • Properties of long-lived daf-2 mutants suggest resistance to organismal death Galimov et al. describe mechanisms of organismal death in C. elegans. They document a rigor mortis-like wave of muscle hyper-contraction accompanied by Ca2+ release and falling ATP, which is coupled to the previously described wave of intestinal necrosis in a process that resembles a distorted and deadly defecation cycle. |
| Databáze: | OpenAIRE |
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