| Autor: |
E W, Kabiru, C M, Mbogo, S K, Muiruri, J H, Ouma, J I, Githure, J C, Beier |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Journal of the American Mosquito Control Association. 13(3) |
| ISSN: |
8756-971X |
| Popis: |
The number of salivary gland malaria sporozoites (sporozoite load) was determined by hemacytometer counts for 2,055 field-collected Anopheles mosquitoes from Kilifi District, Kenya. Of 48 gland-positive Anopheles gambiae s.l., sporozoite loads ranged from 125 to 79,875, with a geometric mean of 1,743 sporozoites per infected mosquito. About half of the infected mosquitoes had sporozoite loads1,000. Following hemacytometer examination of salivary gland samples, the same samples were subsequently tested for Plasmodium falciparum circumsporozoite (CS) protein by enzyme-linked immunosorbent assay (ELISA). The confirmation by ELISA of CS protein in 89.6% (43/48) of the salivary gland-positive samples compared to only 1.4% (28/2,007) of the dissection-negative mosquitoes indicated that dissection methods with hemacytometer counts of sporozoites were adequate for detecting even low numbers of sporozoites in field-collected mosquitoes. Detection of 17 or fewer sporozoites in blood meals of 7 freshly bloodfed An. gambiae s.l. provides a further indication that the actual number of sporozoites transmitted during bloodfeeding may be quite low. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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