| Autor: |
I J, Forbes, P D, Zalewski, C, Giannakis, W H, Betts |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Biochemistry international. 22(4) |
| ISSN: |
0158-5231 |
| Popis: |
Putative binding sites for zinc are present in the regulatory domain of protein kinase C but a distinct role for zinc has not yet been proposed. Here we show that micromolar concentrations of zinc chloride cause pure rat brain protein kinase C to localize in a detergent-insoluble, cytoskeletal fraction of red cell membranes and to bind to isolated cytoskeleton in the presence of phosphatidylserine. Attachment of protein kinase C to cytoskeleton was accompanied by enhanced expression of binding sites for 3H-phorbol ester, a regulatory ligand of protein kinase C. The active factor in the cytoskeleton was labile to protease suggesting that protein kinase C binds to a cytoskeletal protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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