Proteome analysis for 3T3-L1 adipocyte differentiation
| Autor: | Md, Atiar Rahman, Suresh G, Kumar, Sung Hak, Lee, Hee Sun, Hwang, Hyun Ah, Kim, Jong Won, Yun |
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| Rok vydání: | 2009 |
| Předmět: |
Analysis of Variance
Adipogenesis Proteome Blotting Western Proteins Reproducibility of Results Peptide Mapping Mice Gene Expression Regulation 3T3-L1 Cells Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Adipocytes Ribose-Phosphate Pyrophosphokinase Animals Electrophoresis Gel Two-Dimensional |
| Zdroj: | Journal of microbiology and biotechnology. 18(12) |
| ISSN: | 1017-7825 |
| Popis: | Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its control. Therefore, we performed a differential proteome mapping strategy using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3-L1 pre-adipocytes in response to an adipogenic cocktail. In the current study, we identified 46 differentially expressed proteins, 6 of which have not been addressed previously in 3T3-L1 cell differentiation. Notably, we found that phosphoribosyl pyrophosphate synthetase (PRPS), a regulator of cell proliferation, was preferentially expressed in pre-adipocytes than in fully differentiated adipocytes. In conclusion, our results provide valuable information for further understanding of the adipogenic process. |
| Databáze: | OpenAIRE |
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