Recombinant reverse transcriptase of Rous sarcoma virus: characterization of DNA polymerase and RNAase H activities

Autor:	A P, Chernov, A A, Mel'nikov, I I, Fodor
Rok vydání:	1991
Předmět:	DNA Replication Dose-Response Relationship Drug Ribonuclease H Magnesium Chloride Temperature RNA-Directed DNA Polymerase DNA-Directed DNA Polymerase Hydrogen-Ion Concentration In Vitro Techniques Rifamycins Recombinant Proteins Potassium Chloride Avian Sarcoma Viruses Sodium Fluoride Zidovudine
Zdroj:	Biomedical science. 2(1)
ISSN:	0955-9701
Popis:	Enzyme preparations of Rous sarcoma virus (RSV) reverse transcriptase have been isolated from a culture of E. coli HB101(pMF14). The enzyme has been purified to homogeneity and been shown to consist of two subunits, of molecular mass 97.4 and 61.3 kDa, respectively. The optimum conditions for the DNA polymerase and RNAase H activities, fidelity of DNA synthesis on a homogeneous RNA template, and the inhibitory effect of azidothymidine triphosphate have been determined. Data on the use of RSV recombinant reverse transcriptase for cDNA synthesis are given.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4eda0ba4da5d22e5c53b3c21550639a6 https://pubmed.ncbi.nlm.nih.gov/1717011 Zobrazit plný text záznamu