IL-4 enhances IL-10 gene expression in murine Th2 cells in the absence of TCR engagement
| Autor: | C B, Schmidt-Weber, S I, Alexander, L E, Henault, L, James, A H, Lichtman |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Mice
Inbred BALB C Receptors Antigen T-Cell Mice Transgenic Janus Kinase 2 Protein-Tyrosine Kinases Th1 Cells Tyrphostins Interleukin-10 Up-Regulation Mice Mice Inbred AKR Th2 Cells Adjuvants Immunologic Gene Expression Regulation Proto-Oncogene Proteins Cyclosporine Animals Interleukin-2 Interleukin-4 RNA Messenger Cells Cultured |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 162(1) |
| ISSN: | 0022-1767 |
| Popis: | Both IL-4 and IL-10 are regulatory cytokines produced by Th2 cells that can down-regulate cell-mediated immune responses. The studies reported here examine the influence of various cytokines in the regulation of T cell IL-10 production. The results indicate that IL-10 gene expression by TCR transgenic Th2 cells is significantly up-regulated by IL-4 in the absence of TCR signals. IL-4 enhances both IL-10 mRNA levels and secreted protein, and this effect is not related to enhanced mRNA stability. TCR-mediated IL-10 gene expression is inhibited by cyclosporin A, but IL-4-mediated IL-10 expression is not. IL-4 also enhances IL-13 mRNA levels, to a lesser extent than IL-10, but does not significantly effect the expression of other cytokine mRNAs. Furthermore, IL-4 does not significantly enhance IL-10 expression in Th1 cells. IL-2 also enhances effector cytokine production in the absence of TCR signals, but in a subset nonspecific manner, increasing both Th2 IL-4 mRNA and Th1 IFN-gamma mRNA. These data suggest that Th2 IL-4 production may contribute to the down-regulation of immune responses by directly enhancing Th2 IL-10 production. In addition, the data clearly demonstrate that exogenous cytokines can significantly influence effector cytokine production by effector T cells without the requirement for TCR signals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|