| Autor: |
P Ia, Arenkov, E N, Zhmareva, V A, Berezin |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1. 61(6) |
| ISSN: |
0201-8470 |
| Popis: |
The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase. The purified rabbit immunoglobulin G is immobilized on the surface of membranes as the first layer. The competitive immunoassay is employed. The immunoglobulin G concentration range is 1-1000 ng/ml. The membranes with the immobilized antigen can be repeatedly used after incubation in 0.1 M glycine buffer, pH 2.5. The dry membrane with the immobilized antigen can be used after keeping for 6 months in refrigerator at 4 degrees C without changing the concentration range measured. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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