Cannabinoids activate p38 mitogen-activated protein kinases through CB1 receptors in hippocampus
| Autor: | P, Derkinderen, C, Ledent, M, Parmentier, J A, Girault |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Mice Knockout Mitogen-Activated Protein Kinase Kinases Cannabinoids MAP Kinase Kinase 4 Receptors Drug JNK Mitogen-Activated Protein Kinases Hippocampus p38 Mitogen-Activated Protein Kinases Rats Enzyme Activation Rats Sprague-Dawley Mice Cannabinoid Receptor Modulators Animals Lysophospholipids Mitogen-Activated Protein Kinases Phosphorylation Receptors Cannabinoid |
| Zdroj: | Journal of neurochemistry. 77(3) |
| ISSN: | 0022-3042 |
| Popis: | Cannabinoid receptors (CB1-R) are the target of a novel class of neuromodulators, the endocannabinoids. Yet, their signalling mechanisms in adult brain are poorly understood. We report that, in rat and mouse hippocampal slices, anandamide and 2-arachidonoylglycerol, synthetic cannabinoids, and delta(9)-tetrahydrocannabinol activated p38 mitogen-activated protein kinases (MAPK), but not c-Jun N-terminal kinase (JNK). In contrast, lysophosphatidic acid (LPA), a lipid messenger acting on different receptors, increased both p38-MAPK and JNK phosphorylation. The effects of cannabinoids on p38-MAPK were mediated through activation of CB1-R because they were blocked in the presence of SR 141716 A and absent in CB1-R knockout mice, two conditions that did not alter the effects of LPA. The activation of p38-MAPK by cannabinoids was insensitive to inhibitors of SRC: These results provide new insights into the cellular mechanisms by which cannabinoids exert their effects in hippocampus. |
| Databáze: | OpenAIRE |
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