| Autor: |
Micha, Bayer, Jenny A, Morris, Clare, Booth, Allan, Booth, Niki, Uzrek, Joanne R, Russell, Robbie, Waugh, Pete E, Hedley |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1900 |
| ISSN: |
1940-6029 |
| Popis: |
Exome capture is a reduced representation approach that selectively captures sequence from only the gene-bearing regions of a genome. It is based on probes targeted at these regions and, compared with whole genome shotgun sequencing, leads to a significant reduction in cost and data processing effort while still providing insights into the most relevant part of a genome. An exome capture array for barley was released in 2013 and this has opened the door to numerous studies that have put this technology to good use. In this chapter we detail the laboratory protocols required for enrichment and sequencing, and provide detailed step-by-step instructions for the bioinformatics analysis of the resulting data. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
