[Selection of DNA aptamers, specifically interacting with fibrillar form of the yeast Sup35 protein]

Autor:	E R, Surina, E V, Morozkina, E V, Marchenko, M D, Ter-Avanesian, S V, Benevolenskiĭ
Rok vydání:	2009
Předmět:	Saccharomyces cerevisiae Proteins Prions SELEX Aptamer Technique DNA Single-Stranded Saccharomyces cerevisiae Aptamers Nucleotide Peptide Termination Factors
Zdroj:	Molekuliarnaia biologiia. 43(4)
ISSN:	0026-8984
Popis:	Single-stranded DNA aptamers interacting with fibers formed by the Sup35 protein of Saccharomyces cerevisiae were obtained by the SELEX procedure. Specificity of interaction with Sup35p is investigated for 10 from total of 40 selected aptamers. It is shown that 9 aptamers bind to fibrillar Sup35p and not to monomeric form of the protein. The rate of dissociation constant of aptamer-fiber complex varies from 0.1 to 1 microM for different aptamers. Selected aptamers can be used to study prionization of Sup35.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4d9098356aa762fd4a1a2a52609300e6 https://pubmed.ncbi.nlm.nih.gov/19807032 Zobrazit plný text záznamu