| Autor: |
Takahisa, Nishiyama, Erika, Hasegawa, Shigeru, Yanagi, Yoshihisa, Kudo, Ryuta, Hamada, Noriko, Matsumura, Mikiko, Tomino, Yukio, Muromachi, Kiyoshi, Hatakeyama, Hiroyuki, Uchino |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Acta neurochirurgica. Supplement. 118 |
| ISSN: |
0065-1419 |
| Popis: |
We developed a conventional imaging method to measure Ca(2+) concentration in cytosol (using FuraRed as an indicator) and mitochondria (using Rhod-2 as an indicator), simultaneously, by alternative excitation with specific wave length. After confirming the availability of the method in Hela cells, we applied it to mouse whole-brain slice -preparation, which was exposed to oxygen- and glucose-deprived artificial cerebrospinal fluid (ischemic ACSF) for 12 min. The fluorescence (570 nm) at the cerebral cortex and hippocampus due to FuraRed (excited by 480 ± 10 nm) decreased (indicating the increase in cytosolic Ca(2+)-concentration), while the fluorescence due to Rhod-2 (excited by 560 ± 10 nm) increased (indicating the increase in mitochondrial Ca(2+) concentration) during exposure to ischemic conditions. We found the characteristic protective effects of cyclosporine A (10(-6) M), a known blocker for mitochondrial permeability transition, and SEA0400 (10(-6) M), a blocker for Na(+)/Ca(2+) exchanger, on the abnormal Ca(2+) increase in cytosol. We confirmed that the present method will be useful for future pathological and pharmacological studies on ischemia-induced brain damage. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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