Gamma delta T cells respond directly to pathogen-associated molecular patterns
| Autor: | Jodi F, Hedges, Kirk J, Lubick, Mark A, Jutila |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Lipopolysaccharides Transcription Genetic Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Receptors Antigen T-Cell gamma-delta Peptidoglycan Lymphocyte Activation Chemotaxis Leukocyte Animals Newborn T-Lymphocyte Subsets Animals Humans Cattle Chemokines Cells Cultured Oligonucleotide Array Sequence Analysis |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 174(10) |
| ISSN: | 0022-1767 |
| Popis: | Gammadelta T cells recognize unprocessed or non-peptide Ags, respond rapidly to infection, and localize to mucosal surfaces. We have hypothesized that the innate functions of gammadelta T cells may be more similar to those of cells of the myeloid lineage than to other T cells. To begin to test this assumption, we have analyzed the direct response of cultured human and peripheral blood bovine gammadelta T cells to pathogen associated molecular patterns (PAMPs) in the absence of APCs using microarray, real-time RT-PCR, proteome array, and chemotaxis assays. Our results indicate that purified gammadelta T cells respond directly to PAMPs by increasing expression of chemokine and activation-related genes. The response was distinct from that to known gammadelta T cell Ags and different from the response of myeloid cells to PAMPs. In addition, we have analyzed the expression of a variety of PAMP receptors in gammadelta T cells. Freshly purified bovine gammadelta T cells responded more robustly to PAMPs than did cultured human cells and expressed measurable mRNA encoding a variety of PAMP receptors. Our results suggest that rapid response to PAMPs through the expression of PAMP receptors may be another innate role of gammadelta T cells. |
| Databáze: | OpenAIRE |
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