| Autor: |
María S, Relloso, Jimena, Nievas, Santiago, Fares Taie, Victoria, Farquharson, María T, Mujica, Vanesa, Romano, Mariela S, Zarate, Jorgelina, Smayevsky |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Revista Argentina de microbiologia. 47(2) |
| ISSN: |
0325-7541 |
| Popis: |
The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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