[Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification]

Autor: María S, Relloso, Jimena, Nievas, Santiago, Fares Taie, Victoria, Farquharson, María T, Mujica, Vanesa, Romano, Mariela S, Zarate, Jorgelina, Smayevsky
Rok vydání: 2014
Předmět:
Zdroj: Revista Argentina de microbiologia. 47(2)
ISSN: 0325-7541
Popis: The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification.
Databáze: OpenAIRE