Methods to detect antifibrillarin antibodies in patients with systemic sclerosis (SSc): a comparison
| Autor: | Josefina Huerta, García, Monica Delgado, Osuna, Filiberto Martinez, Castrejon, Laura Guzman, Enriquez, Pedro A, Reyes, J Jesus Cortes, Hermosillo |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Scleroderma
Systemic integumentary system urogenital system Chromosomal Proteins Non-Histone fungi Blotting Western Original Articles Immunologic Tests Precipitin Tests Antibody Specificity Humans RNA Small Nucleolar skin and connective tissue diseases Fluorescent Antibody Technique Indirect Autoantibodies HeLa Cells |
| Zdroj: | Journal of clinical laboratory analysis. 18(1) |
| ISSN: | 0887-8013 |
| Popis: | Autoantibodies against nucleolar antigens are common in systemic sclerosis (SSc). They include autoantibodies against fibrillarin (Fb), which are serological markers for SSc. Fb is associated with the evolutionally‐conserved box C/D of small nucleolar RNAs (snoRNAs). We compared indirect immunofluorescence (IIF), Western blot (WB), and immunoprecipitation (IPP) of total small RNAs assays to determine which of these techniques is most specific for the detection of snoRNPs. We also examined the frequency and specificity of autoantibodies from SSc patients to snoRNAs, snRNAs, and scRNAs, and concluded that 1) IIF can not determine autoantibody specificity against Fb, 2) 36% of SSc sera were false‐negative by WB, and 3) by IPP, anti‐Fb autoantibodies from SSc patients can bind U3, U8, U13, U15, and U22 snoRNAs. J. Clin. Lab. Anal. 18:19–26, 2004. © 2004 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|