Inhibition of protein kinase C by semisynthetic phospholipid plasmanyl-(N-acyl)-ethanolamine, a nontoxic antitumor preparation

Autor:	I S, Mikhaevich, G K, Gerasimova, J, Kára
Rok vydání:	1991
Předmět:	Cell Extracts Kinetics Mice Mice Inbred C3H Binding Sites Animals Brain Phospholipid Ethers Antineoplastic Agents Female Phosphatidylserines Chromatography DEAE-Cellulose Protein Kinase C
Zdroj:	Biochemistry international. 23(2)
ISSN:	0158-5231
Popis:	Protein kinase C was extracted from mouse brain and partially purified by ion-exchange chromatography on a DEAE-cellulose column. Its activity was determined by incorporation of phosphate from [gamma-32P]ATP into histone H2b. The semisynthetic alkyl-phospholipid plasmanyl-(N-acyl)-ethanolamine (PNAEs) with selective antitumor activity inhibited the activity of the protein kinase in a cell-free system in the presence of phosphatidylserine, a protein kinase C activator. The inhibition was competitive with respect to phosphatidylserine, the inhibition constant being 40 microM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4cfc0a3dc0d86412c769f9fbe6cc11a7 https://pubmed.ncbi.nlm.nih.gov/1859429 Zobrazit plný text záznamu