Spontaneous CD4
| Autor: | Hayden, Pearce, Paul, Hutton, Shalini, Chaudhri, Emilio, Porfiri, Prashant, Patel, Richard, Viney, Paul, Moss |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male endocrine system Enzyme-Linked Immunospot Assay Adolescent Cancer testis antigens CD8-Positive T-Lymphocytes Lymphocyte Activation MAGE Interferon-gamma Young Adult Clinical Testicular Neoplasms Testicular cancer Antigens Neoplasm Humans T cells Research Article|Clinical Membrane Proteins Neoplasms Germ Cell and Embryonal Tumor immunology Peptides Immunologic Memory Orchiectomy Research Article |
| Zdroj: | European Journal of Immunology |
| ISSN: | 1521-4141 |
| Popis: | Cancer/testis antigen (CTAg) expression is restricted to spermatogenic cells in an immune‐privileged site within the testis. However, these proteins are expressed aberrantly by malignant cells and T‐cell responses against CTAgs develop in many cancer patients. We investigated the prevalence, magnitude and phenotype of CTAg‐specific T cells in the blood of patients with testicular germ cell tumors (TGCTs). CD8+ and CD4+ T‐cell responses against MAGE‐A family antigens were present in 44% (20/45) of patients’ samples assayed by ex vivo IFN‐γ ELISPOT. The presence of MAGE‐specific CD8+ T cells was further determined following short‐term in vitro expansion through the use of pMHC‐I multimers containing known immunogenic peptides. Longitudinal analysis revealed that the frequency of MAGE‐specific T cells decreased by 89% following orchidectomy suggesting that persistence of tumor antigen is required to sustain CTAg‐specific T‐cell immunity. Notably, this decrease correlated with a decline in the global effector/memory T‐cell pool following treatment. Spontaneous T‐cell immunity against CTAg proteins therefore develops in many patients with testicular cancer and may play an important role in the excellent clinical outcome of patients with this tumor subtype. |
| Databáze: | OpenAIRE |
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