| Autor: |
Bach-Nga, Pham, Michèle, Roussel, Thierry, Peyrard, Marylise, Beolet, Véronique, Jan-Lasserre, Dominique, Gien, Maryline, Ripaux, Sébastien, Bourgouin, Sandrine, Kappler-Gratias, Philippe, Rouger, Pierre-Yves, Le Pennec |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Transfusion. 51(12) |
| ISSN: |
1537-2995 |
| Popis: |
Whether anti-D produced by individuals with a weak D phenotype are allo- or autoantibodies remains a matter of debate even though blood transfusion practice is impacted. The aim of our study was to determine the serologic features of anti-D in individuals expressing the most frequent weak D type in Caucasians that are weak D Type 1 or weak D Type 2, to assess whether anti-D were allo- or autoantibodies.Serologic D typing and molecular analysis enabled the including of 121 weak D Type 1 individuals and 99 weak D Type 2 individuals in our study. Serologic features of anti-D included autologous controls, direct antiglobulin test, elution, and titration of anti-D before and after adsorption of serum on autologous red blood cells (RBCs).Serologic D typing showed a variable reactivity of RBCs expressing weak D Type 1 or weak D Type 2 (4+ to 0). Anti-D was identified in six weak D Type 1 and six weak D Type 2 individuals, respectively. The serologic data were in favor of autoantibodies.A complete anti-D investigation in individuals with a D variant (weak D or partial D identified by molecular analysis) should be systematically performed before any valid conclusion on the nature of the antibody. Transfusing weak D Type 1 or weak D Type 2 patients with D+ RBC units should be recommended. Weak D Type 1 or weak D Type 2 pregnant women do not need anti-D immunoprophylaxis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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