| Autor: |
V, Natarajan, S, Smeland, J O, Moskaug, S, Kamiyama, S, Masushige, S, Kato, R, Blomhoff |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
European journal of cell biology. 73(4) |
| ISSN: |
0171-9335 |
| Popis: |
The most prominent differences between mammalian and non-mammalian vertebrate retinol-binding proteins (RBP) are in the C-terminal sequences. We have cloned and sequenced the cDNA for chicken RBP. Transfected COS cells that transiently expressed mammalian (human) or non-mammalian (chicken) RBP were used to demonstrate that both proteins were able to bind retinol and human transthyretin. However, we observed an increased retinol-independent secretion in cells expressing chicken RBP and reduced ligand-dependent secretion compared to the human protein. It can therefore be concluded that the C-terminal amino acid tail which is missing in chicken RBP compared to human RBP might play a role in retention and ligand-induced secretion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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