| Autor: |
Q-F, Wei, J-S, Yao, Y-T, Yang |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
European review for medical and pharmacological sciences. 23(17) |
| ISSN: |
2284-0729 |
| Popis: |
Osteosarcoma (OS) is a common malignant bone tumor that poses a serious threat to the health of adolescents or children. A large number of studies have proposed the role of microRNAs (miRNAs) in OS, except for miR-1247. Therefore, this research was designed to explore the molecular mechanism of miR-1247 in OS.Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) or Western blot analysis was used to measure the expressions of miR-1247 and genes. The function of miR-1247 was investigated using Cell Counting Kit-8 (CCK-8) and transwell assays. The Dual-Luciferase reporter assay was used to explore the relationship between miR-1247 and neuropilin-1 (NRP1).MiR-1247 was downregulated in OS, which was related to the aggressive behavior of OS patients. Moreover, miR-1247 inhibited cell viability and metastasis in OS. At the same time, miR-1247 promoted apoptosis and inactivated the Wnt/β-catenin pathway in OS. Furthermore, it was confirmed that NRP1 was a direct target of miR-1247. Upregulation of NRP1 attenuated the inhibitory effect of miR-1247 in OS.MiR-1247 played a suppressive role in OS by suppressing cell viability and metastasis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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