Ectopic expression of myc or myn down-regulates immunoglobulin transcription
| Autor: | M, Sigvardsson, K, Johansson, L G, Larsson, K, Nilsson, T, Leanderson |
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| Rok vydání: | 1994 |
| Předmět: |
B-Lymphocytes
Mice Inbred BALB C Genes Immunoglobulin Transcription Genetic Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Genes myc Down-Regulation Gene Expression Immunoglobulins Mice Transgenic DNA-Binding Proteins Proto-Oncogene Proteins c-myc Immunoglobulin kappa-Chains Mice Animals Promoter Regions Genetic Transcription Factors |
| Zdroj: | Leukemia. 8(7) |
| ISSN: | 0887-6924 |
| Popis: | Co-transfection of expression vectors for c-myc or myn down-regulated the expression from a reporter plasmid containing the chloramphenicol-acetyl-transferase (CAT) gene, under the control of an immunoglobulin kappa promoter and the immunoglobulin heavy-chain enhancer. The effect was dose-dependent and an additive effect was seen when both expression vectors were transfected simultaneously. Deletion mutants lacking the leucine zipper region of c-myc were unable to down-regulate the transcription from the reporter construct. Reporter genes where the immunoglobulin enhancer had been exchanged with a SV40 enhancer or an immunoglobulin minimal enhancer were still down-regulated by a cotransfection with c-myc expression vectors. A minimal promoter containing an octamer motif responded to myc expression while a minimal promoter containing a SP1 motif did not. No direct interactions between myc, myn and Oct proteins could be detected either by band-shift analysis, or by co-translation in vitro followed by precipitation with SP6 kappa promoter bound to magnetic carriers. Furthermore, B cells from mice transgenic for myc showed aberrant expression of transcription factors. |
| Databáze: | OpenAIRE |
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