Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System

Autor:	John A, Calarco, Ari E, Friedland
Rok vydání:	2015
Předmět:	Animals Genetically Modified Genome INDEL Mutation Gene Targeting Animals CRISPR-Cas Systems Cloning Molecular Caenorhabditis elegans Genetic Engineering Plasmids RNA Guide Kinetoplastida
Zdroj:	Methods in molecular biology (Clifton, N.J.). 1327
ISSN:	1940-6029
Popis:	The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4b93b7eb94e40cb14135e68f7b212348 https://pubmed.ncbi.nlm.nih.gov/26423968 Zobrazit plný text záznamu