Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System
Autor: | John A, Calarco, Ari E, Friedland |
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Rok vydání: | 2015 |
Předmět: | |
Zdroj: | Methods in molecular biology (Clifton, N.J.). 1327 |
ISSN: | 1940-6029 |
Popis: | The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome. |
Databáze: | OpenAIRE |
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