Comparison of the types of mutations induced by 7,12-dimethylbenz[a]anthracene in the lacI and hprt genes of Big Blue rats

Autor:	R A, Mittelstaedt, M G, Manjanatha, S D, Shelton, L E, Lyn-Cook, J B, Chen, A, Aidoo, D A, Casciano, R H, Heflich
Rok vydání:	1998
Předmět:	Hypoxanthine Phosphoribosyltransferase 9 10-Dimethyl-1 2-benzanthracene Escherichia coli Proteins DNA Mutational Analysis Rats Inbred F344 Rats Animals Genetically Modified Repressor Proteins Bacterial Proteins Carcinogens Lac Repressors Animals Point Mutation Lymphocytes Transgenes
Zdroj:	Environmental and molecular mutagenesis. 31(2)
ISSN:	0893-6692
Popis:	An important question regarding the use of transgenic reporter genes to detect mutation in rodents is how the types of mutations recovered in transgenes compare with the types of mutations found in endogenous genes. In this study, we examined mutations induced by 7,12-dimethylbenz[a]anthracene in the lacI transgene and the endogenous hprt gene of lymphocytes from Big Blue rats and in the hprt gene of lymphocytes from nontransgenic Fischer 344 rats. The overall mutation profiles found in these genes were remarkably similar: the majority of mutations were base pair substitutions, with the most common mutation being A:T--T:A transversion. Differences were found for the mutational profiles in the endogenous gene and transgene with respect to the location of the mutations and the orientation of basepair substitutions in the DNA strands. In most cases, these differences could be explained by the nature of the target genes. The results support the use of the lacI transgene for detecting in vivo mutation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4b7c2a731a869d2f82ca9fc826631ef2 https://pubmed.ncbi.nlm.nih.gov/9544192 Zobrazit plný text záznamu Plný text