Characterization of an antibody directed against a 128 kDa glycoprotein involved in the thrombogenicity of the elastin-associated microfibrils
| Autor: | F, Fauvel-Lafeve, P, Picard, G, Godeau, Y J, Legrand |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Extracellular Matrix Proteins
Platelet Aggregation Macromolecular Substances Immune Sera Immunoblotting Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Muscle Smooth Vascular Elastin Immunoenzyme Techniques Contractile Proteins Immunoglobulin G Endothelium Vascular RNA Splicing Factors Thrombospondins Blood Coagulation Cells Cultured Glycoproteins Research Article |
| Zdroj: | The Biochemical journal. 255(1) |
| ISSN: | 0264-6021 |
| Popis: | We have developed a monospecific antiserum directed against a major glycoprotein in the elastin-associated microfibrils with an apparent molecular mass of 128 kDa (GP 128). When immunoblotting or enzyme-linked immunosorbent microassay was used, its IgGs recognized thrombospondin in a platelet lysate, but did not react with several basement-membrane-derived macromolecules, nor with plasma fibronectin. Similar patterns of immunofluorescence and immunoperoxidase were found after incubation of endothelial cells with either anti-GP 128 or anti-(platelet thrombospondin) IgGs. Both antibodies inhibited the microfibrils- and thrombin-induced platelet aggregation, and were without effect on the aggregation by other inducers. These results confirm that there is an antigenic homology between GP 128 and thrombospondin. |
| Databáze: | OpenAIRE |
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