| Autor: |
A, van Heerden, K S, Browning |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
The Journal of biological chemistry. 269(26) |
| ISSN: |
0021-9258 |
| Popis: |
The subunits (p28 and p86) of the isoenzyme form of eukaryotic initiation factor 4F (eIF-(iso)4F) from wheat were expressed separately in Escherichia coli. The subunits were purified by affinity chromatography (p28) and ion-exchange chromatography (p86). The purified subunits alone did not support polypeptide synthesis in an eIF-(iso)4F and eIF-4F-deficient translation system from wheat germ. However, when the two subunits were mixed together, activity equal to that of the native form of eIF-(iso)4F was obtained. These results show that subunits expressed separately are able to associate and form an enzymatically active complex. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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