| Autor: |
G M, Pollack, R L, Slaughter, J F, Buchanan, D D, Shen |
| Rok vydání: |
1984 |
| Předmět: |
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| Zdroj: |
Journal of chromatography. 311(1) |
| Popis: |
A high-performance liquid chromatographic procedure was developed for the determination of di-(2-ethylhexyl)phthalate (DEHP) concentrations in human whole blood samples. The solvent extraction of DEHP was found to be highly variable between samples obtained from different subjects (coefficient of variation of 30.4%). The recovery of DEHP following extraction with ethyl acetate was negatively correlated with serum lipid content, as expressed by the sum of serum cholesterol and triglyceride concentrations (r = -0.864). The technique of standard addition of DEHP allowed a single-point calibration of DEHP extractability in individual blood samples, and provided an accurate estimation of DEHP concentration (coefficient of variation of approximately 6% in replicate samples). The potential for intersample variability in the solvent extraction of other highly lipid-soluble compounds should be considered. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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