Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre
| Autor: | Katie J, Grayson, Kaitlyn M, Faries, Xia, Huang, Pu, Qian, Preston, Dilbeck, Elizabeth C, Martin, Andrew, Hitchcock, Cvetelin, Vasilev, Jonathan M, Yuen, Dariusz M, Niedzwiedzki, Graham J, Leggett, Dewey, Holten, Christine, Kirmaier, C, Neil Hunter |
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| Rok vydání: | 2016 |
| Předmět: |
Light
Photosystem I Protein Complex Spectrum Analysis fungi Blotting Western Pilot Projects macromolecular substances Rhodobacter sphaeroides Models Biological Article Luminescent Proteins Microscopy Electron nervous system Bacterial Proteins Microscopy Fluorescence Fluorescence Resonance Energy Transfer Quantum Theory Photosynthesis |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Photosynthesis uses a limited range of the solar spectrum, so enhancing spectral coverage could improve the efficiency of light capture. Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the cytoplasmic side of the RC complex. Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP–RC intermolecular distance and spectral overlap between the emission of YFP and the visible-region (QX) absorption bands of the RC allow energy transfer via a Förster mechanism, with an efficiency of 40±10%. This proof-of-principle study demonstrates the feasibility of increasing spectral coverage for harvesting light using non-native genetically-encoded light-absorbers, thereby augmenting energy transfer and trapping in photosynthesis. Photosynthesis uses only a limited range of solar radiation. Here, Grayson et al. genetically incorporated the yellow fluorescent protein (YFP) chromophore into a bacterial photosystem, and show that energy harvested by reaction centre–YFP complexes can augment photosynthesis in vivo. |
| Databáze: | OpenAIRE |
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