| Autor: |
Toshio, Ohshima, Saori, Nomiya, Yukari, Yamamoto, Miki, Miyazawa, Jun, Ohsuga, Akifumi, Hisada, Kenji, Iwawaki, Satomi, Asai, Hayato, Miyachi |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
The Tokai journal of experimental and clinical medicine. 42(1) |
| ISSN: |
2185-2243 |
| Popis: |
Antimicrobial susceptibility testing for fastidious bacteria, such as Haemophilus influenzae (H. influenzae) and Streptococcus pneumoniae (S. pneumoniae) has been performed manually. We evaluated the performance of a newly developed fully automated system for rapid bacterial identification and antimicrobial susceptibility testing "RAISUS ANY" (Nissui Pharmaceutical Co., Ltd.).We evaluated the performance of "RAISUS ANY" for measurement of minimal inhibitory concentrations (MICs) of H. influenzae and S. pneumoniae, in comparison with the manual method (DP34, Eiken Chem. Co., Ltd.). The repeatability of MICs was studied using the reference strain of these bacteria, obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).The comparison with the manual method for 35 and 36 clinical strains of H. influenzae and S. pneumonia showed 62.9-100% and 86.1-100% agreement, respectively. Five of 35 H. influenzae strains that showed a trailing effect were stably and accurately measured for MICs without a variation among the examiners.In conclusion, the automated system "RAISUS ANY" provided a reliable MICs data for H. influenzae and S. pneumonia, suggesting its improvement in performance and reliability for routine antimicrobial susceptibility testing in clinical bacteriological laboratories. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
