The role of depolarizing activation of Na

Autor: Ningning, Li, Ruilou, Zhu, Shuang, Zeng, Yangyang, Wang, Yitian, Yang, Ningning, Fu, Mengrong, Miao, Mingyang, Sun, Jiaqiang, Zhang
Rok vydání: 2022
Předmět:
Zdroj: Life sciences. 308
ISSN: 1879-0631
Popis: The aim of this study was to investigate the role of depolarizing activation of NaOn postnatal days 7, 8, and 9, mice were exposed to 3 % sevoflurane for 2 h per day. The proliferation, differentiation, and myelin sheath of OPC were observed with immunofluorescence, quantitative real-time polymerase chain reaction (QRT-PCR), and transmission electron microscopy (TEM) at various time points. The open field, Y maze, and new object recognition tests were used to measure spatial learning and memory. siRNA was used for the knockdown NCX1 in human OPC (HOPC) before sevoflurane exposure; the Transwell migration assay was used to measure cell migration ability and Fluo 4-AM was used to measure intracellular Ca2+ concentration.Pretreatment with an NCX inhibitor attenuated the proliferation and differentiation of OPC induced by sevoflurane and induced a remarkable increase in platelet-derived growth factor receptor-alpha (PDGFRα), 2, 3-cyclic nucleotide 3-phosphodiesterase (CNPase), oligodendrocyte transcription factor 2 (Olig2), and homeodomain protein NK2 homeobox 2 (NKX2.2) levels. Pretreatment with an NCX inhibitor alleviated the sevoflurane-induced myelination disorder and cognitive impairment. The decreased cell migration and increased intracellular CaThis study suggests that repeated sevoflurane exposure in newborn mice leads to depolarization of OPC, which leads to Ca
Databáze: OpenAIRE
Externí odkaz: