Expression of TGF-beta and its receptors in murine fetal and adult dermal wounds
| Autor: | A J, Cowin, T M, Holmes, P, Brosnan, M W, Ferguson |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Wound Healing Receptor Transforming Growth Factor-beta Type I Receptor Transforming Growth Factor-beta Type II Fluorescent Antibody Technique Gene Expression Regulation Developmental Protein Serine-Threonine Kinases Transforming Growth Factor beta1 Mice Transforming Growth Factor beta2 Transforming Growth Factor beta3 Gene Expression Regulation Transforming Growth Factor beta Animals Wounds and Injuries Female RNA Messenger Activin Receptors Type I Receptors Transforming Growth Factor beta In Situ Hybridization Skin |
| Zdroj: | European journal of dermatology : EJD. 11(5) |
| ISSN: | 1167-1122 |
| Popis: | The transforming growth factor-betas (TGF-betas) are of major importance in wound healing and have been implicated in the scar-less wound repair observed in fetuses. Few studies have characterised the role of TGF-beta in fetal wound repair and to date no studies have characterised the expression of its receptors within non-scarring fetal wounds. We have localised the TGF-beta isoforms beta1, beta2 and beta3 and its two receptors, TGF-betaRI and TGF-betaRII in both adult and fetal dermal murine wounds. We observed low level immunofluorescence of TGF-beta1 and TGF-beta2 in fetal wounds and although TGF-beta3 staining was observed in the epidermis of fetal skin, there was no upregulation in response to injury. By contrast, all three isoforms were strongly expressed in adult wounds. Similar to its ligands, TGF-beta receptor expression was increased post-wounding in the adult wounds. However, in contrast, no mRNA or protein for either of the TGF-beta receptors was observed in response to wounding in the fetal dermis although there was both mRNA and protein expression of both the receptors localised within the fetal alimentary tract, one of the few fetal organs which does scar post-injury. The differences that we observed in the expression of TGF-beta and its receptors in adult and fetal wounds could be important in the absence of scar formation that is observed in the fetus. |
| Databáze: | OpenAIRE |
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