Regulation of atrial natriuretic peptide clearance receptors in mesangial cells by growth factors
| Autor: | R V, Paul, P S, Wackym, M, Budisavljevic, E, Everett, J S, Norris |
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| Rok vydání: | 1993 |
| Předmět: |
DNA Replication
Base Sequence Molecular Sequence Data Blotting Northern Kidney Binding Competitive Polymerase Chain Reaction Culture Media Serum-Free Glomerular Mesangium Rats Up-Regulation Kinetics Radioligand Assay Oligodeoxyribonucleotides Animals RNA Messenger Receptors Atrial Natriuretic Factor Atrial Natriuretic Factor Cells Cultured Thymidine |
| Zdroj: | The Journal of biological chemistry. 268(24) |
| ISSN: | 0021-9258 |
| Popis: | Rat mesangial cells can express both 130-kDa guanylyl cyclase-coupled and 66-kDa non-coupled atrial natriuretic peptide (ANP) receptors (ANPR-A and ANPR-C, respectively). Exposure of mesangial cells, grown in 20% fetal calf serum, to 0.1% serum for 24 h increased total ANP receptor density more than 2-fold (Bmax = 87 versus 37 fmol/mg of cell protein) without changing binding affinity (Kd = 94 versus 88 pM). Radioligand binding and cross-linking studies demonstrated that up-regulation of ANP binding after serum deprivation was entirely due to an increase in ANPR-C, with little or no change in ANPR-A. Inhibition of protein synthesis with cycloheximide blocked up-regulation after serum deprivation. Steady-state ANPR-C mRNA level was increased 15-fold by serum deprivation, as judged by Northern blotting. There was no change in ANPR-A mRNA. Platelet-derived growth factor and phorbol myristate acetate, when added to low serum medium, blocked or reversed the effect of serum deprivation on ANPR-C. We conclude that synthesis and expression of ANPR-C but not ANPR-A is suppressed by serum, platelet-derived growth factor, and phorbol myristate acetate. Suppression of ANPR-C in vivo could contribute to mesangial cell proliferative responses to growth factors. |
| Databáze: | OpenAIRE |
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