A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

Autor:	Kevin K, Ariën, Guido, Vanham, Youssef, Gali
Rok vydání:	2011
Předmět:	CD4-Positive T-Lymphocytes Virus Cultivation Anti-HIV Agents Cell Survival Cell Culture Techniques Cell Differentiation Epithelial Cells HIV Infections Dendritic Cells Genitalia Female Models Biological Coculture Techniques Cell Line High-Throughput Screening Assays HIV-1 Humans Female
Zdroj:	Current protocols in cell biology.
ISSN:	1934-2616
Popis:	Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4a496b99ee2536379b24a3713e0ce9bb https://pubmed.ncbi.nlm.nih.gov/21898337 Zobrazit plný text záznamu Plný text