In vitro PET evaluations in lung cancer cell lines
| Autor: | T, Xing, F, Wu, O, Brodin, K J, Fasth, B, Långström, M, Bergström |
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| Rok vydání: | 2000 |
| Předmět: |
Carbon Isotopes
Antibiotics Antineoplastic Lung Neoplasms Diazooxonorleucine Biological Transport Diagnosis Differential Levodopa Evaluation Studies as Topic Fluorodeoxyglucose F18 Carcinoma Non-Small-Cell Lung Tumor Cells Cultured Humans Bromine Radioisotopes Carcinoma Small Cell Drug Monitoring Radiopharmaceuticals Floxuridine Tomography Emission-Computed |
| Zdroj: | Anticancer research. 20(3A) |
| ISSN: | 0250-7005 |
| Popis: | One purpose of the study was to explore the PET tracer 11C-L-DOPA for the discrimination between small-cell lung cancer (SCLC) and non small-cell lung cancer (NSCLC). A further aim was to explore the potential antitumoral effects of 6-diazo-5-oxy-L-norleucine (DON) and the use of a PET proliferation marker for the evaluation.Four lung cancer and one endocrine tumour cell line (BON) were cultured as monolayer. The uptake of 5-[76Br]-bromo-2-fluoro-deoxyuridine (76Br-BFU), [11C]-L-DOPA (11C-DOPA) and [18F]-fluorodeoxyglucose (18FDG) were evaluated. The effects of specific enzyme inhibitors affecting the DOPA metabolism were explored. The effect of DON on proliferation and uptake of 76Br-BFU were assessed.All cell types showed a measurable uptake of 11C-DORA, with slightly lower values in lung cancer. There were no clear differences between SCLC and NSCLC. The addition of COMT inhibitor induced a significantly increased uptake of the tracer in BON cells, but not in lung cancer cells. DON significantly reduced the proliferation in all cell lines. The 76Br-BFU uptake was reduced markedly in all cell lines during DOn treatment.11C-DOPA failed to distinguish between SCLC and NSCLC. DON showed strong antiproliferative effects which might motivate renewed interest in this drug for clinical cancer treatment. PET with 76Br-BFU might be used for treatment evaluation. |
| Databáze: | OpenAIRE |
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