Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion
| Autor: | C M, Buchanan, A R, Phillips, G J, Cooper |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
endocrine system Secretory Vesicles Molecular Sequence Data Down-Regulation Cell Separation In Vitro Techniques Antibodies Peptide Fragments Cell Line Rats Perfusion Islets of Langerhans Mice Glucose Insulin-Like Growth Factor II Insulin Secretion Animals Humans Insulin Amino Acid Sequence Protein Precursors Rats Wistar Research Article |
| Zdroj: | The Biochemical journal. 360(Pt 2) |
| ISSN: | 0264-6021 |
| Popis: | Pancreatic islet beta-cells secrete the hormones insulin, amylin and pancreastatin. To search for further beta-cell hormones, we purified peptides from secretory granules isolated from cultured murine beta TC6-F7 beta-cells. We identified a 34-amino-acid peptide (3948 Da), corresponding to Asp(69)-Leu(102) of the proinsulin-like growth factor II E-peptide, which we have termed 'preptin'. Preptin, is present in islet beta-cells and undergoes glucose-mediated co-secretion with insulin. Synthetic preptin increases insulin secretion from glucose-stimulated beta TC6-F7 cells in a concentration-dependent and saturable manner. Preptin infusion into the isolated, perfused rat pancreas increases the second phase of glucose-mediated insulin secretion by 30%, while anti-preptin immunoglobulin infusion decreases the first and second phases of insulin secretion by 29 and 26% respectively. These findings suggest that preptin is a physiological amplifier of glucose-mediated insulin secretion. |
| Databáze: | OpenAIRE |
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