[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)]

Autor: Bo, Zhang, Min-ren, Huang, Qiang, Zhuge, Zheng-min, Han, Tong-ming, Yin, Hui-xin, Pan, Li-huang, Zhu, Rong-ling, Wu, Ming-xiu, Wang
Rok vydání: 2005
Zdroj: Yi chuan = Hereditas. 24(5)
ISSN: 0253-9772
Popis: DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv."Lux"(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible). Out of 114 RAPD primers, four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.
Databáze: OpenAIRE