Overexpression of a transmembrane isoform of neural cell adhesion molecule alters the invasiveness of rat CNS-1 glioma
| Autor: | G C, Owens, E A, Orr, B K, DeMasters, R J, Muschel, M E, Berens, C A, Kruse |
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| Rok vydání: | 1998 |
| Předmět: |
Brain Neoplasms
Genetic Vectors Metalloendopeptidases Glioma Flow Cytometry Transfection Rats Retroviridae Matrix Metalloproteinase 9 Cell Movement Gelatinases Rats Inbred Lew Animals Humans Matrix Metalloproteinase 2 Neoplasm Invasiveness Collagenases Neural Cell Adhesion Molecules Cell Division DNA Primers |
| Zdroj: | Cancer research. 58(9) |
| ISSN: | 0008-5472 |
| Popis: | CNS-1 is a highly invasive neural cell adhesion molecule (NCAM)-positive rat glioma that exhibits similarities in its pattern of infiltration to human gliomas. To investigate whether increasing NCAM expression alters invasive behavior, retroviruses encoding human NCAM 140 and a cytoplasmic truncation of NCAM 140 were used to transduce a population of CNS-1 glioma cells that had a relatively low endogenous level of NCAM. Compared to cells transduced with a control virus, cells overexpressing either intact or truncated human NCAM 140 showed decreased invasion of a reconstituted basal lamina. Changes in growth rate or in key matrix metalloproteinase activities could not account for this result. In a migration assay on type IV collagen, cells exhibited a substrate concentration-dependent increase in the rate of migration; however, overexpression of NCAM 140 or truncated NCAM 140 inhibited motility at higher substrate concentrations. Consistent with these findings was the decreased spread of NCAM 140 overexpressers in vivo following instillation of cells into the right frontal cortex of rat brain. NCAM 140 overexpressers showed considerably more restricted perivascular and periventricular spread than cells transduced with a control virus. However, NCAM-140-overexpressing tumor exhibited a less cohesive pattern of growth near the site of tumor instillation and more individual cell infiltration of brain parenchyma with more pronounced perineuronal satellitosis. The stability of recombinant NCAM expression was confirmed by recovering tumor cells from tumor-bearing animals and measuring NCAM levels by flow cytometry. These observations show that overexpression of NCAM 140 decreases the long-range spread of CNS-1 glioma along basal lamina pathways but enhances local infiltration of neuropil. |
| Databáze: | OpenAIRE |
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