| Autor: |
Marie M, Darby, Kedra, Wallace, Denise, Cornelius, Krystal T, Chatman, Janae N, Mosely, James N, Martin, Christine A, Purser, Rodney C, Baker, Michelle T, Owens, B Babbette, Lamarca |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Medical journal of obstetrics and gynecology. 1(2) |
| ISSN: |
2333-6439 |
| Popis: |
To investigate a role of Vitamin D in the pathogenesis of preeclampsia (PE), and to discern any potential benefits of Vitamin D supplementation on hypertension in the RUPP rat model of PE.Blood and placentas from normal pregnancies (NP) and PE were collected following elective cesarean delivery without evidence of infection. Circulating Vitamin D was extracted by HPLC and measured via mass spectrometry. Media for placenta explants was supplemented with Vitamin D and exposed to hypoxic (1% O2) or normoxic (6% O2) conditions for 24 hours. ELISAs were performed on media and normalized to total protein to determine cytokine secretion. RUPP rats were supplemented with vitamin D by oral gavage, and blood pressure (MAP) and pup weights were measured in NP and RUPP rats with or without Vitamin D supplementation. Flow cytometry was used to evaluate CD4Inflammatory cytokine secretion was higher (p0.05) while the anti-inflammatory cytokine, IL-10, was significantly lower in the media of PE placentas compared to NP (p=0.005). Vitamin D supplementation decreased hypoxia stimulated pro-inflammatory cytokine secretion (p=0.003) in the media of PE placentas. Vitamin D decreased MAP and circulating CD4Vitamin D supplementation may be useful in the treatment or prevention of hypertensive disorders in pregnancy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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