| Autor: |
S C, Suffin, A F, Kaufmann, B, Whitaker, K B, Muck, G A, Prince, D D, Porter |
| Rok vydání: |
1980 |
| Předmět: |
|
| Zdroj: |
Archives of pathologylaboratory medicine. 104(6) |
| ISSN: |
0003-9985 |
| Popis: |
A modified glucose oxidase immunoenzyme technique was shown to be highly sensitive and specific for detection of serogroup 1 Legionella pneumophila in 4% formaldehyde solution-fixed, paraffin-embedded tissue sections. There was complete concordance between infection with L pneumophila and detection of the organisms in tissue sections by glucose oxidase immunoenzyme staining. The L pneumophila organisms stained blue-black and were found within phagocytic cells as well as in the extracellular space. A cloud of blue-black pigment, probably representing diffusable antigen, was present in the extracellular spaces in the area of L pneumophila localization. No false-positive or false-negative reactions were found. This technique requires no specialized equipment, may be applicable to retrospective diagnostic problems, and can be adapted to routine diagnostic practice. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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