Effect of inactivated
| Autor: | Aki, Miyauchi, Wataru, Watanabe, Toshi, Akashi, Seiko, Hashiguchi, Hiroki, Yoshida, Chihiro, Sugita, Masahiko, Kurokawa |
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| Rok vydání: | 2018 |
| Předmět: |
BALF
bronchoalveolar lavage fluids Streptococcus pneumonia TiO2 titanium dioxide S. pneumoniae Streptococcus pneumoniae ISP inactivated S. pneumoniae PBS phosphate-buffered saline PFU plaque-forming units RSV ELISA enzyme-linked immunosorbent assay Pneumonia CFU colony-forming units Article Non-pathogenic pneumococcal particles IFN interferon RSV respiratory syncytial virus Infiltrated cells ComputingMethodologies_COMPUTERGRAPHICS |
| Zdroj: | Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • We made inactivated Streptococcus pneumoniae (ISP) as non-pathogenic particles. • We evaluated effects of ISP on development of pneumonia by RSV infection in mice. • ISP didn’t show histopathological effects on lungs of RSV-infected mice. • ISP reduced virus titer and infiltration of lymphocyte in the lungs. • The inherent activity of ISP as particles in RSV infection is discussed. The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivated Streptococcus pneumoniae (ISP) as non-pathogenic particles on the severity of pneumonia in RSV-infected mice. Mice were intranasally exposed to ISP before RSV infection. On day 5 post-infection, we examined tissues, virus titer, and infiltrated cells in the lungs. The ISP did not cause significant histopathological effects in the lungs of RSV infected mice, but reduced virus titer. It also reduced the ratio of lymphocyte infiltration into the lungs and consequently the ratio of macrophage increased. In addition, we found that ISP increased RANTES level in bronchoalveolar lavage fluid from RSV-infected mice on day 1 post-infection, but reduced type I interferon levels. Thus, ISP did not exacerbate pneumonia in RSV infection, rather, it might mildly reduce the severity. We characterize and discuss the inherent activity of ISP as non-pathogenic particles inducing the role of RANTES on the pneumonia in RSV infection. |
| Databáze: | OpenAIRE |
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