IL-1 and TNF-alpha are important factors in the pathogenesis of murine recurrent herpetic stromal keratitis
| Autor: | T L, Keadle, N, Usui, K A, Laycock, J K, Miller, J S, Pepose, P M, Stuart |
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| Rok vydání: | 2000 |
| Předmět: |
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha Corneal Stroma Enzyme-Linked Immunosorbent Assay Mice Inbred Strains Herpesvirus 1 Human Up-Regulation Virus Shedding Mice Recurrence Immunoglobulin G Keratitis Herpetic Animals Female Virus Activation RNA Messenger DNA Primers Interleukin-1 |
| Zdroj: | Investigative ophthalmologyvisual science. 41(1) |
| ISSN: | 0146-0404 |
| Popis: | To better understand the role of interleukin (IL)-1 and tumor necrosis factor (NF)alpha in recurrent herpetic stromal keratitis (HSK), the cytokine content and the effects of anti-cytokine antibodies on mouse corneas with the disease were examined.Competitive reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent analyses of IL-1alpha and TNF-alpha content were performed on corneas removed 3, 5, 7, 10, 14, and 21 days after latently infected NIH mice were irradiated with UV-B light to reactivate herpes simplex virus (HSV). In separate experiments, mice were injected with anti-IL-1 or anti-TNF-a antibodies 1 day before and 7 days after reactivation.UV-B irradiation stimulated an increase in corneal IL-la mRNA in reactivated (virus shedding) mice. This increase persisted longer and was higher than in UV-B irradiated uninfected control animals. IL-1alpha and TNF-alpha protein in corneas of reactivated mice was significantly elevated on days 3 to 10 compared with day 0 levels, and exceeded levels in control corneas on the same days. Anti-IL-1 and anti-TNF-alpha antibody administration both resulted in significantly decreased virus-induced corneal opacity between 7 and 21 days after UV-B exposure.IL-1alpha and TNF-alpha are upregulated in corneas in mice experiencing recurrent HSK. Abrogation of virus-induced corneal disease by anti-cytokine antibodies suggests that these cytokines play important roles in the pathogenesis of recurrent disease. Therefore, neutralization of specific proinflammatory cytokines may have potential therapeutic value. |
| Databáze: | OpenAIRE |
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