| Autor: |
Qing-Wei, Zhou, Jing-Li, Xie, Li, Xin, Ren, Xu, Qing, Ye, Zai-Ping, Li, Ren-Bao, Gan |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 35(2) |
| ISSN: |
0582-9879 |
| Popis: |
The cDNA encoding Kringle 1-4 and part of Kringle 5 domains of human plasminogen (K1-4.5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1-4.5 was transformed into Pichi pastoris GS115 and the recombinant yeast was induced to express the recombinant proteins by methanol. The expressed proteins were purified by lysine affinity chromatography to a purity of 95%. The recombinant K1-4.5 inhibited the growth of bovine capillary endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner with a half maximal concentration of 2 mg/L. rhK1-4.5 also inhibited 40% of the BAEC migration stimulated by bFGF in the concentration of 1 mg/L. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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