| Autor: |
Clotilde B, Angelucci, Roberto, Giacominelli-Stuffler, Mauro, Maccarrone |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2576 |
| ISSN: |
1940-6029 |
| Popis: |
Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid (AA) and ethanolamine. The method described here measures FAAH activity through the fluorometric arachidonoyl-7-amino-4-methyl-coumarin amide (AAMCA) substrate, which allows a simple and sensitive assay suitable for high-throughput screening tests. FAAH catalyzes the hydrolysis of AAMCA producing AA and the highly fluorescent compound 7-amino-4-methylcoumarin (AMC). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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