[Effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells]
| Autor: | Hao, Wang, Sheng-shun, Tan, Xin-yang, Wang, Dong-hua, Liu, Chun-shui, Yu, Zhuan-li, Bai |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Blotting Western Antineoplastic Agents Flow Cytometry Inhibitor of Apoptosis Proteins Neoplasm Proteins Gene Expression Regulation Neoplastic Cell Line Tumor Humans RNA Small Interfering Melanoma Adaptor Proteins Signal Transducing |
| Zdroj: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 23(8) |
| ISSN: | 1007-8738 |
| Popis: | To investigate the effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells.Three chemically synthesized specific siRNAs targeting to livin were transfected to LiBr cells, then the expression of livin was detected both at mRNA level by real-time RT-PCR and at protein level by Western blot, and the effective one was selected for use on observation of dose- and time-response to livin silencing.One of three designed siRNAs could effectively knock down the livin expression both at mRNA and protein levels in dose- and time- dependent manners, 100 nmol/L of which achieved the highest knockdown effect on mRNA at 48 h and on protein at 72 h after transfection.Expression of livin in LiBr cells could be efficiently knocked down by siRNA with target site 790-808 of livin mRNA. |
| Databáze: | OpenAIRE |
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