Laboratory evidence of active HIV-1 infection in Canadians with hemophilia associated with administration of heat-treated factor VIII

Autor:	P, Neumann, M, O'Shaughnessy, R, Remis, C, Tsoukas, D, Lepine, M, Davis
Rok vydání:	1990
Předmět:	Canada Factor VIII Hot Temperature Immunoblotting Gene Amplification Enzyme-Linked Immunosorbent Assay HIV Infections Hemophilia A Polymerase Chain Reaction DNA Viral HIV Seropositivity HIV-1 Humans Longitudinal Studies
Zdroj:	Journal of acquired immune deficiency syndromes. 3(3)
ISSN:	0894-9255
Popis:	Heat-treated factor VIII has been implicated in the transmission of HIV to hemophiliacs. Previously, evidence has been limited to documenting cases of seroconversion following administration of heat-treated factor VIII. Here, we present evidence of active HIV infection, i.e., infected and not merely sensitized following factor VIII injections. Six Canadians with hemophilia had seroconverted during a longitudinal study of their HIV immune status. Two of the three patients tested by this method demonstrated HIV gag-specific sequences upon amplification by polymerase chain reaction. In addition, HIV-1 virus was isolated from peripheral blood lymphocytes of one of these two persons as shown by reverse transcriptase activity of culture supernatants as well as neutralizable p24 antigen. This, we believe, is the first evidence of active HIV infection following administration of 60 degrees C, 30 h heat-treated factor VIII.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::480b62882015c4bbf38f478e5d6a8efb https://pubmed.ncbi.nlm.nih.gov/2106024 Zobrazit plný text záznamu