| Autor: |
Shizuo, Kayama, Raita, Yano, Katsutoshi, Yamasaki, Chiemi, Fukuda, Keiko, Nishimura, Hitoshi, Miyamoto, Hiroki, Ohge, Motoyuki, Sugai |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Journal of microbiological methods. 148 |
| ISSN: |
1872-8359 |
| Popis: |
Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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